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. 2020 Jul 21;11:1539. doi: 10.3389/fmicb.2020.01539

FIGURE 4.

FIGURE 4

Amylase and cellulase activity assays of AmyE1 and CelE1. (A) PhoA-fusion assay (the lower panel) demonstrated that AmyE1 and CelE1 are secreted into the periplasm as computationally predicted (two upper panels). The two upper panels are computational prediction of signal peptide for AmyE1 and CelE1 secretion by using the SignalP 5.0. The 5′-nucleotide sequence, encoding the signal peptide of amyE1, celE1, xlnC, xlnA, and licA gene, was fused with the E. coil phoA gene without the sequence encoding the N-terminal signal peptide, respectively. (B) Photographic representation of the SDS-PAGE of AmyE1 proteins. Lane 1: marker proteins; lane 2: enzyme supernatant (amylase). Molecular weights were presented in the form of kDa. The amylase activity was defined under the assay conditions described. (C) Photographic representation of the SDS-PAGE of CelE1 proteins. Lane 1: marker proteins; lane 2: enzyme supernatant (cellulase). Molecular weights were presented in the form of kDa. The cellulase activity was defined under the assay conditions described.