TABLE 1.
Plasmids used in this study.
| Plasmids | Description | Source or reference |
| pUCP20T | Shuttle vector Ampr | H.P. Schweizer |
| pUCP20T-phoA(SP) | The E. coli phoA gene cloned in pUCP20T | This study |
| pUCP20T-phoA(NSP) | The modified phoA gene [phoA(NSP)], without the 5′-sequence encoding the N-terminal signal peptide, cloned in pUCP20T | This study |
| pUCP20T-amyE1(SP)-phoA(NSP) | The amyE1-phoA fusion gene, with the 5′-sequence encoding the N-terminal signal peptide fused with phoA(NSP), cloned in pUCP20T | This study |
| pUCP20T-celE1(SP)-phoA(NSP) | The celE1-phoA fusion gene, with the 5′-sequence encoding the N-terminal signal peptide fused with phoA(NSP), cloned in pUCP20T | This study |
| pUCP20T-xlnC(SP)-phoA(NSP) | The xlnC-phoA fusion gene, with the 5′-sequence encoding the N-terminal signal peptide fused with phoA(NSP), cloned in pUCP20T | This study |
| pUCP20T-xlnA(SP)-phoA(NSP) | The xlnA-phoA fusion gene, with the 5′-sequence encoding the N-terminal signal peptide fused with phoA(NSP), cloned in pUCP20T | This study |
| pUCP20T-licA(SP)-phoA(NSP) | The licA-phoA fusion gene, with the 5′-sequence encoding the N-terminal signal peptide fused with phoA(NSP), cloned in pUCP20T | This study |
| pET28a | Expression vector with T7lac promoter | Novogene |
| pET28a-amyE1 | Overexpression construct of amyE1 | This study |
| pET28a-celE1 | Overexpression construct of celE1 | This study |
| pMK3 | E. coli–Bacillus shuttle plasmid; ampicillin resistance in E. coli and kanamycin resistance in B. subtilis | |
| pMK3-amyE1 | The amyE1 gene cloned in pMK3 | This study |
| pMK3-celE1 | The celE1 gene cloned in pMK3 | This study |