FIGURE 9.

Antibacterial and antibiofilm activity of phage P545. (A) P545 inhibited the formation of biofilms. P545 was incubated with CRKP strain KP4 at MOIs of 0.1, 0.01, and 0.001 in 96-well plates for 24 h. (B) P545 disrupted the mature biofilm of KP4. Biofilm formation of KP4 was induced in 96-well plates for 24 h, and then the biofilm was treated with P545 at MOIs of 0.1, 0.01, and 0.001 for 24 h. Fresh TSB medium was used as a negative control. The OD₅₉₅ values of the residual biofilms were measured after crystal violet staining. All the data are shown as the mean ± SEM. ***indicates p < 0.001.