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. 2020 Jul 21;11:1117. doi: 10.3389/fphar.2020.01117

Figure 1.

Figure 1

Representative whole slide images of Masson’s trichrome stained section of the left lung with enlarged details (scale bar 100 μm) at 14 days after BLM treatment with IT or OA protocols and saline respectively (A). The total fibrosis quantification based on Ashcroft (B). The data represent the mean ± s.e.m. of seven animals for each time point per group. Changes in BLM groups were compared to the saline group using two-way ANOVA followed by Dunnett’s test. *p <0.05; **p <0.01. Time course of frequency distribution of Ashcroft scores divided in three classes as (1–3) mild, 4 moderate, ≥5 severe fibrosis after BLM treatment with IT or OA protocols and saline respectively (C). Ashcroft score among the five lobes at 14 days for IT (D) and OA BLM administration (E). Left lobe (Sx); right: cranial (Dx Cr), middle (Dx M), caudal (Dx Ca), accessory lobe (Dx Ac). Data distribution of seven animals for each time point per group are graphed as box plot and the median values are linked through a light blue line. Kruskall–Wallis test was performed followed by Dunn’s multiple comparison test to compare Ashcroft score among the lung lobes. *p <0.05; **p <0.01.