Figure 1.

PcrA lethality is suppressed by recJ, recO, or recA inactivation, but not by addAB, recQ, rarA, recX, or recU inactivation. Log phase cultures of wt, single and double mutant strains were diluted and plated on LB agar containing 500 μM IPTG (yellow bars) or lacking it (gray bars). Lethality assays showing cell viability upon PcrA depletion in recJ, recO, or recA cells (A) and in addAB, recQ, rarA, recX, or recU cells (B). Log phase cultures of indicated strains were diluted and plated on LB agar containing IPTG and 0.2 mM H₂O₂ (blue bars) or IPTG and 1.3 mM MMS (green bars). Experiments were performed at least four times. The dotted lines mark the survival rate upon PcrA depletion. Data are shown as mean fractional survival ± SEM.