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. 2020 Jul 21;11:1696. doi: 10.3389/fmicb.2020.01696

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Copyright © 2020 Fan, Li, Zhao, Liu, Liu, Wang, Dong, Wang, Chi, Song, Sun, Wang, Ha, Zhao, Bao, Wu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The sensitivity and specificity of recombinased-based amplification. ASFV DNA molecules after series dilution were detected by real-time RPA (A) and RAA (B) (copies per reaction). Probit regression analysis using MedCalc Software was performed on data of 8 replicates from serial dilutions by real-time RPA (C) and RAA (D). Specificity test result of real-time RPA (E) and RAA (F) on detecting ASFV 24 genotypes, CSV, FMDV, PRV, PCV2, and PPRSV.