FIGURE 2.

Overexpression of SLC12A5 promotes BUC invasion and metastasis in vitro and in vivo. A, B, qRT‐PCR and western blotting showing the relative expression of SLC12A5 mRNA (A) and protein (B) in several BUC cell lines and normal bladder uroepithelial cell line SV‐HUV‐1. C, D, qRT‐PCR and western blotting showing the relative expression of SLC12A5 mRNA (C) and protein (D) after overexpressing SLC12A5 in 5637 and UM‐UC‐3 cell lines. E, F, Transwell migration assays showing that overexpression of SLC12A5 promoted the migration (E) and invasion (F) of 5637 and UM‐UC‐3 cell lines. G, Tail vein injection lung metastatic models. Number of tumors was compared between the SLC12A5‐vector group and the SLC12A5‐overexpressing group. n = 6. N, normal lung; M, lung metastatic tumor; **P < .01