Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 20;111(7):2598–2607. doi: 10.1111/cas.14435

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

Cancer stem cells in Epstein‐Barr virus (EBV)‐associated gastric cancer are enriched for CD44v6/v9^+/+ cells. A, Spheroid formation ability of TMK1 and EBV⁺ TMK1 cells. Upper panels, representative images of spheroid colonies derived from TMK1 and EBV⁺ TMK1 cells (7 days). Lower panels, number and diameter of the spheres (mean ± SD; **P < .01, Mann‐Whitney U test). B, Spheroid formation assay of CD44v6/v9^+/+ and CD44v6/v9^−/− fractioned cells of SNU719 cells. A total of 10 000 cells were seeded in each well. Measurement of the number (left) and diameter (right) of spheroid colonies after 10 days (mean ± SD; *P < .05, **P < .01, Mann‐Whitney U test). C, Spheroid formation assay of CD44v6/v9^+/+ and CD44v6/v9^−/− fractioned cells of EBV⁺ TMK1 cells. A total of 500 cells were seeded in each well. Measurement of the number (left) and diameter (right) of spheroid colonies after 10 days (mean ± SD; *P < .05, **P < .01, Mann‐Whitney U test). D, Tumor formation of CD44v6/v9^+/+ and CD44v6/v9^−/− cells from SNU719 cells in vivo. A total of 90 000 cells embedded in Matrigel were inoculated s.c. into SCID mice (n = 10 per group). Measurement of the tumor volume after 67 days (red arrows indicate tumors derived from CD44v6/v9^+/+ cells). Lower graph shows volume (mean ± SEM). E, Tumor formation of CD44v6/v9^+/+ and CD44v6/v9^−/− cells from EBV⁺ TMK1 in vivo. A total of 150 000 cells embedded in Matrigel were inoculated s.c. into SCID mice (n = 5 per group). Measurement of tumor volumes after 34 days (blue arrows indicate tumors derived from CD44v6/v9^−/− cells, and red arrows indicate tumors derived from CD44v6/v9^+/+ cells). Lower graph shows tumor volume (mean ± SEM; P = .0079, Mann‐Whitney U test)