FIGURE 3.

Gelsolin (GSN) knockdown results in enhanced proliferation and invasion ability through glycogen synthase kinase‐3β (GSK3β). A, Transfected cells were stained and counted to determine the invasion ability. *P < .05; **P < .01; ***P < .001 vs negative control (NC), 1‐way ANOVA. B, Proliferation was determined by CCK‐8 analysis and measured by optical density (OD⁴⁵⁰) value at 0, 24, 48, and 72 h. **P < .01; ***P < .001 vs NC, 2‐way ANOVA. C, After transfection with siGSN (20 nmol/L), the expression of GSN, GSK3β, phosphorylated GSK3β serine9 (p‐GSK3β^Ser9), and phosphorylated GSK3β tyrosine 216p‐(GSK3β^Tyr216) was determined. Band intensity was measured