Figure 2.

Selection experiment comparing phenotypic selection and MAS from a potato population segregating for drought tolerance. (A) Distribution of tuber dry weight in segregating population G1 before selection for above average yield (~600 lines) and population G2 after selection for yield (~200 lines) (experiment P1, yield from three plants, AR = offspring from cross A_t×R_s, EA = offspring from cross E_s×A_t) (B) Scheme comparing procedure and timeline for phenotypic, yield-based selection of subpopulation PP_t, and MAS of subpopulations MP_t selected for superior tolerance and MP_s selected for inferior tolerance from 200 G2 lines. Duration of tasked written in red is fixed, duration of those written in black depends on resources. For detailed project plan see Data Sheet 1 , Figure S1 . (C) Venn diagram showing the number of lines in each subpopulation and the overlap between the subpopulations. There is no overlap between the marker selected populations MP_t and MP_s. (D) Tuber starch yield of the phenotypically selected PP_t compared to the remaining lines of G2 grown under optimal (c) and reduced (s) water supply in big-bag/pot (B2, P3) and field trials (F2) with significant water stress. Different letters indicate significant differences between population within an experiment and treatment (REGWQ-Test, α = 0.05). (E) Drought tolerance predicted from metabolite concentrations for parent cultivars and G2 lines from the crosses A_t×R_s (red) and E_s×A_t (green).