Figure 1.

Rv1507A induces pro-inflammatory cytokines. RAW264.7 cells were treated with purified Rv1507A protein (2, 5, 10 μg/ml). LPS was used as positive control while PBS and heat inactivated (HI) protein served as negative controls. Levels of IL-12 (A), IL-6 (B), and TNF-α (C) were estimated using ELISA, as described in methods. Representative data from three experiments show the concentration of IL-12, IL-6, and TNF-α as mean ± SEM. Statistical significance was determined with one-way ANOVA. Additionally, spleen was recovered from BALB/c mice (n = 7) that were injected with Ms_Vc (1 × 10⁷) or Ms_Rv1507A (1 × 10⁷). Primed splenocytes were cultured in-vitro in presence of Rv1507A protein. Culture supernatants were harvested after 12 h, 24 h, and 48 h and concentrations of IL-12 (D), IL-6 (E), and TNF-α (F) were determined. Representative data from triplicate wells show the concentration of IL-12, IL-6, and TNF-α as mean ± SEM. Statistical significance was determined with two-way ANOVA. P < 0.05 was considered significant, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (nd-not detected).