Figure 4.

Rv1507A protein and recombinant Ms_Rv1507A mount effector and central memory like response. Spleens were recovered from BALB/c mice that were either injected with purified Rv1507A proteins (10 μg/ml) or PBS alone. Splenocytes were cultured in presence of Rv1507A, in-vitro for 96 h. Representative density plots of CD8⁺CD44^highCD62L^high (T_CM) cells and CD4⁺CD44^highCD62L^high cells are shown (A). CD8⁺CD44^high (T_EM) cells and CD4⁺CD44^high cells are shown in (B). Additionally, splenocytes from mice infected with Ms_Vc (1 × 10⁷) or Ms_Rv1507A (1 × 10⁷) were re-stimulated with Rv1507A in-vitro for 96 h. Percent of CD8⁺ cells expressing CD44^highCD62L^high (C) and CD44^high cells (D) were measured by flow cytometry, shown as mean ± SEM. Similarly CD4⁺ T_CM and T_EM were quantified and depicted in (E,F), respectively. Statistical significance was determined with one tailed Mann–Whitney test. p < 0.05 was considered significant, *p < 0.05, **p < 0.01.