Figure 8.

Ms_Rv1507A increases the infiltration of lymphocytes in the lung tissue of immunized mice. Lungs were recovered after 30 days from BALB/c mice (n = 6) that were injected with either Phosphate buffer saline (un-infected) or Ms_Vc (1 × 10⁷) or Ms_Rv1507A cells (1 × 10⁷) intratracheally. Lungs were washed in PBS and then fixed in 10% formalin solution. After fixation, lungs were fine–sectioned and stained with Hematoxylin and Eosin (HE) solution. Eosin is pink and stains proteins non-specifically. In a typical tissue, nuclei are stained blue, whereas the cytoplasm and extracellular matrix have varying degrees of pink staining. The images were captured for at least 5 different fields in 6 mice.