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. 2020 Jul 21;14:223. doi: 10.3389/fncel.2020.00223

FIGURE 1.

FIGURE 1

Electro-acupuncture promotes the release of exosomal miR-146b in peri-ischemic striatum. (A) The expression of exosome-associated markers HSP70, TSG101, and CD81 were analyzed by Western blotting. (B) The expression of markers were quantified on the basis of average band intensity (n = 4). (C) MiRNA microarray analysis of exosomes from peri-ischemic striatum and clustering of 25 significantly altered miRNAs with ≥1.5-fold difference in expression between MCAO and MCAO+EA groups. (D) Four miRNAs were significantly upregulated and 1 miRNA were downregulated in exosomes of the MCAO+EA group compared with the MCAO group were checked by RT-qPCR (n = 3). (E) The expression of miR-146b in tissues of peri-ischemic striatum were analyzed form MCAO, MCAO+miR-146b inhibitors, MCAO+EA, MCAO+EA+miR-146b inhibitors groups (n = 3). Data represent means ± SD of the results of representative experiment. *P< 0.05, MCAO vs. Sham; **P < 0.01, MCAO vs. Sham; #P< 0.05 and ##P< 0.01, MCAO vs. MCAO+EA; &&P< 0.01, MCAO vs. MCAO+miR-146b inhibitors; MCAO+EA vs. MCAO+EA+miR-146b inhibitors.