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Purification of lipase from PLS 80.
| Purification | Volume (mL) | Protein |
Enzyme |
Purity (fold) | Yield (%) | |||
|---|---|---|---|---|---|---|---|---|
| Concen-tration (mg/mL) | Total (mg) | Activity (U/mL) | Specific Activity (U/mg) | Total Activity (U) | ||||
| Crude enzyme | 1200 | 0.651 | 781.2 | 35.3 | 54.2 | 4236 | 1 | 100 |
| Ammonium Sulphate (40–60%) |
8.4 | 0.166 | 1.39 | 48.1 | 289.8 | 404.4 | 5.34 | 0.95 |
| Sepharose CL-6B | 10 | 0.101 | 1.01 | 33.0 | 326.6 | 329.9 | 6.02 | 0.78 |
