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[Preprint]. 2020 Jul 16:3652322. [Version 1] doi: 10.2139/ssrn.3652322

Figure 2. White pulp attrition, early loss of germinal centers and Bcl-6 expressing B cells in COVID-19 spleens.

Figure 2.

(A) Cross-sectional view of whole spleen and hematoxylin–eosin staining from early (left) and late (right) COVID-19 patients. (B) Low-power images of CD3 (red), CD19 (green), Bcl-6 (orange) and DAPI (blue) staining in a spleen from late COVID-19 patient (left) and control (right). (C) Representative multi-color immunofluorescence image of CD3 (red), CD19 (green), Bcl-6 (orange) and AID (purple) staining in spleens from early (left) and late (middle) COVID-19 patients and controls (right). (D and E) Absolute numbers of CD19+ B cells (D) and CD3+ T cells (E) in spleens from early (purple) (n = 4) and late (blue) (n = 6) COVID-19 patients and controls (green) (n = 7). (F and G) Relative proportion of Bcl-6+ B cells (F) and AID+ B cells (G) among CD19+ B cells in spleens from early (purple) (n = 4) and late (blue) (n = 6) COVID-19 patients and controls (green) (n = 7). SP=Spleen. Multiple comparisons are controlled for by Kruskal-Wallis test. Error bars represent mean ±SEM. *p < 0.05.