Figure 1. Zone 3 in the SEAM eye organoids composed of cells expressing ocular surface ectoderm gene profile.

(A) hESC-derived SEAM organoids were differentiated for 55 days, then processed for single cell RNA-seq and immunohistochemical analysis (n=1 biological replicate). B) Unbiased clustering was conducted using the Seurat Package in R. C) SEAM eye organoids were evaluated for the presence of ocular surface ectoderm markers in Zone 3. D) Cells possessing ocular surface ectoderm annotation were further clustered, presented as UMAP and PCA. E) UMAP presentation of relative expression of known corneal markers across the ocular surface ectoderm cell clusters. F) Heatmap of genes distinguishing each cluster by relative expression. G) Violin plots of known markers of corneal, limbal and conjunctival cells.