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. 2020 Jul 27;10:12491. doi: 10.1038/s41598-020-69500-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HCA2 receptor agonists induce calcium mobilization in bovine neutrophils. (A) Representative figures of the measured fluorescence of neutrophils labeled with Fura-2/AM probe and stimulated with (A) β-hydroxybutyrate (BHB), (B) MK-1903, (C) nicotinic acid and (D) nicotinamide. The arrows indicate the time at which the agonist was added. On the right are the dose response curves of HCA2 agonist-induced calcium influx in bovine neutrophils. The EC₅₀ was calculated with GraphPad Prism v5.0 software.