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. 2020 Jul 27;11(7):593. doi: 10.1038/s41419-020-2726-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Ago2 RIP assay for the amounts of circASPH in A549 and PC9 cells, respectively. The expression levels of circASPH were measured by RT-PCR. c Schematic model shows the potential binding sites of miR-1182, miR-1236, miR-370, and miR-375 on circASPH. d The whole mature sequence of circASPH was cloned into the downstream region of hRluc luciferase reporter gene. Dual-luciferase reporter assay analysis of the effects of miR-1182, miR-1236, miR-370, and miR-375 on circASPH in 293T cells. e, f Biotin-coupled miR-370 captured a fold change of circASPH in the complex as compared with biotin-coupled miR-Scramble. The captured complex from biotin-coupled miRNA capture assays were detected using qRT-PCR and semi-qPCR. g Co-localization between circASPH and miR-370 was observed in A549 and PC9 cells. Nuclei were stained with DAPI (blue). Every cell test was carried out in triplicate and n = 3 for each cell group.