Fig. 1.

Essential protein kinases identified by the functional shRNA screen. (A) The average ranks of 521 protein kinases from the essential shRNA screen performed in UMSCC25, UMSCC8, JHU011, HN6, HN12, and Cal27 cells are graphed along with the 10 murine control genes indicated with green symbols. The ranks for the protein kinases and murine control genes in the individual cell lines are presented in Supplemental Table 1. EGFR, ERBB2, MET, and FGFR1 were not identified as highly essential as single protein kinases and are shown with yellow symbols. Based on the published literature (see text in Results), the top-ranked essential kinases were assembled into interaction networks centered on MTOR (B) and ERBB3 (C) in which the average ranks of the indicated protein kinases among the six HNSCC cell lines are denoted by the colors shown in the color scale bar.