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. 2020 Jul 27;10:12463. doi: 10.1038/s41598-020-69512-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activity of NTAT and CTAT at various pH and temperature: (A) The activities of NTAT and CTAT were carried out at various temperatures and their relative activities were calculated. NTAT displayed activity similar to that of TAT while CTAT was sensitive to temperature variation largely as seen from the plots. (B) The pH stability of NTAT and CTAT was checked at various pH and their activities were plotted in terms of relative activity. NTAT, as seen in temperature profile, remained stable relative to TAT⁹ but CTAT displayed reduced activity at extreme pH conditions.