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. 2020 Jul 27;10:12464. doi: 10.1038/s41598-020-69373-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spruce GTs contain a large diversity of volatile monoterpenes and sesquiterpenes. Isolated GT were collected in a headspace vial and volatiles were sampled with a solid-phase microextraction (SPME) fiber and analyzed by GC–MS using an HP-5 column. GT trichomes volatiles consist mostly of monoterpenes followed by a highly diverse profile of sesquiterpenes. Peaks were identified by a combination of authentic standards, linear retention index calculated with alkane standards, and best library hit using the NIST and Whiley libraries as shown in Table S1.