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. 2020 Jul 27;10:12452. doi: 10.1038/s41598-020-69356-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum-likelihood tree of the new piPolB genes from the LREC dataset. As indicated, strain names are colored based on the phylogenetic group of strains. Previously described pipolin-harboring isolate 3-373-03_S1_C2 was included as a reference. The best-fit model was GTR + F + R2 for all considered criteria by ModelFinder⁶². Scale bar indicates the substitution rate per site.