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. 2020 Jul 27;10:12508. doi: 10.1038/s41598-020-69422-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pain intensity and associated plasma proteins in FM. The Score plot (left) shows a within-group separation in the FM group based on pain intensity (visual analogue scale, VAS). The plot shows that FM patients are grouped as having mild (5–44), moderate (45–74), and severe (75–100) pain intensity. The larger the triangle, the higher the pain intensity. The loading plot (right) shows 13 significant proteoforms (VIP > 1) associated with pain intensity. Nine proteoforms were associated with higher pain intensity in FM (VAS > 45). Numbers in the loading plot equals the spot numbers in Table 3 and supplementary Figure S1 and Table S1. The loading and score plots were created in SIMCA P + (version 15).