Table 3.
Pain intensity and associated plasma proteins in FM.
| Spot number | Protein name | Accession number | VIPpred | p(corr) | FM median OD (interquartile range) | CON median OD (interquartile range) | Fold change FM versus CON |
|---|---|---|---|---|---|---|---|
| Mild pain intensity (5–44) | |||||||
| 6103 | Fibrinogen beta chain | P02675 | 1.21 | -0.67 | 465 (173) | 449 (200) | 1.04 |
| 8508 | Fibrinogen alpha chain | P02671 | 1.15 | -0.64 | 383 (266) | 382 (343) | 1.00 |
| 8402 | Fibrinogen alpha chain | P02671 | 1.12 | -0.62 | 275 (292) | 245 (185) | 1.12 |
| 6203 | Beta-2-glycoprotein 1 | P02749 | 1.06 | -0.58 | 747 (290) | 663 (251) | 1.13 |
| Moderate pain intensity (45–74) | |||||||
| 3810 | Ceruloplasmin | P00450 | 1.31 | + 0.72 | 203 (169) | 216 (174) | 0.94 |
| 5104 | Haptoglobin beta chain | P00738 | 1.18 | + 0.65 | 112 (394) | 226 (349) | 0.50 |
| 3207 | Antithrombin-III | P01008 | 1.15 | + 0.64 | 669 (256) | 687 (257) | 0.97 |
| 4808 | Alpha-2-macroglobulin | P01023 | 1.13 | + 0.63 | 392 (421) | 320 (829) | 1.22 |
| 7404 | Serotransferrin | P02787 | 1.13 | + 0.63 | 350 (308) | 310 (291) | 1.13 |
| 5905 | Alpha-2-macroglobulin | P01023 | 1.07 | + 0.59 | 328 (541) | 455 (388) | 0.72 |
| 6403 | Hemopexin | P02790 | 1.04 | + 0.57 | 1,047 (746) | 1,198 (556) | 0.87 |
| Severe pain intensity (75–100) | |||||||
| 1007 | Immunoglobulin J chain | P01591 | 1.13 | + 0.62 | 405 (489) | 526 (415) | 0.77 |
| 1107 | Clusterin | P10909 | 1.01 | + 0.56 | 1,546 (645) | 1,650 (832) | 0.94 |
Plasma proteins with a VIP > 1, associated with pain intensity in the FM group. Spot numbers refers to equal numbers in Fig. 3 and marked proteins spots in supplementary Figure S1 and Table S1. Accession numbers are according to the protein data base UniProt (www.uniprot.org). Variable influence on projections (VIP) indicates the importance of the regressor, the higher value, the more important for the model. A VIP > 1 is considered as a significant protein. p(corr) is shown and is the correlation coefficient of each variable (protein) in the model. A positive p(corr) for a variable indicates a positive multivariate correlation with the Y-variable (pain intensity), and a negative p(corr), indicates a negative multivariate correlation with pain intensity in FM. The median quantified intensity (optical density, OD) from each group is shown in the table as a comparison. As descriptive values, protein fold change in FM compared to CON is reported. Please note that fold change is a univariate measure and do not necessarily correspond to p(corr); i.e., a positive fold change (FM > CON) is not automatically equal to a positive p(corr). The CON group is not included in the OPLS analysis.