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. 2020 Jul 27;10:12511. doi: 10.1038/s41598-020-69202-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Record of blood flow and wave shape in representative cases. Right side: thumbnail image, Left side: enlarged partial view. The experiment was divided into 4–5 periods according to different stages of treatment in thumbnail image. 1, Baseline: No treatment. 2, Occlusion time: Occlusive suture was placed around the artery, and there was no flow for 7 to 8 min. 3, Thrombus time: The distal clamps were loosened and occlusive thrombi were formed within 20 to 30 min in femoral arteries. 4, Thrombolysis time: different thrombolytic methods were used after forming a stable occlusion. 5, Observation time: the thrombolytic effect was observed after treatment. Black arrow: Using ultrasound to flash.