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. 2020 Jul 27;11(7):583. doi: 10.1038/s41419-020-02796-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The subcellular localization of SH3PXD2A-AS1 in HTR-8/SVneo cell detected using cell fractionation. U6: nucleus marker; GAPDH: cytoplasm marker. b The subcellular localization of SH3PXD2A-AS1 in HTR-8/SVneo detected using FISH. Red: SH3PXD2A-AS1; blue: nucleus. c Proteins interacting with SH3PXD2A-AS1 detected in HTR8/SVneo cell by pulldown and mass spectrometry. d GO terms (Cellular Components) with over-presentation of SH3PXD2A-AS1-binding proteins. e TF-target network for DEG of EOSPE. Red triangles represent transcription factors (TFs) binding SH3PXD2A-AS1; blue circles represent differentially expressed target genes in EOSPE; f overlaps between CTCF target genes (3640) curated in database and differentially expressed genes (3116) detected in the transcriptome sequencing on placentae of patients with EOSPE. g Binding proteins of SH3PXD2A-AS1 detected by RIP assays and qRT-PCR. h SH3PXD2A-AS1 overexpression reduces CTCF target genes. Top 10 target genes with highest expression change in transcriptomes of EOSPE patients were evaluated using qRT-PCR. Left histogram blot shows the results of qRT-PCR. The reduced expression of two targets were verified by western blot (left). i SH3PXD2A-AS1 knockout increases CTCF target genes. Top 10 target genes with highest expression change in transcriptomes of EOSPE patients were evaluated using qRT-PCR. Left histogram blot shows the results of qRT-PCR. The increased expression of two targets were verified by western blot (left). **p < 0.01, *p < 0.05.