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. 2020 Jul 27;11(7):583. doi: 10.1038/s41419-020-02796-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The expression level of CTCF in HTR8/SVneo cells transfected with full-length CTCF. Left: results of qPCR; right: results of western blot. b The expression level of CTCF in HTR8/SVneo cells transfected with shRNA of CTCF. Left: results of qPCR; right: results of western blot. c The reduced expression of SH3PXD2A and CCR7 in HTR8/SVneo cells with CTCF overexpression. Left: results of qPCR; right: results of western blot. d The increased expression of SH3PXD2A and CCR7 in HTR8/SVneo cells with shRNA of CTCF. Left: results of qPCR; right: results of western blot. e CTCF’s binding to the promoters of SH3PXD2A and CCR7 detected by ChIP assays and qPCR in HTR8/SVneo cells. f CTCF’s binding to the promoter of SH3PXD2A-2 detected by ChIP assays with SH3PXD2A-AS1 overexpression or knockout in HTR8/SVneo cells. g CTCF’s binding to the promoter of CCR7-3 detected in HTR8/SVneo cells with SH3PXD2A-AS1 overexpression or knockout using ChIP assays. h The transcription activity of SH3PXD2A-2 and CCR7-3 promoters detected in HTR8/SVneo cells with SH3PXD2A-AS1 overexpression or knockout using luciferase reporter assay. **p < 0.01, *p < 0.05.