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. 2020 Jul 27;11(7):583. doi: 10.1038/s41419-020-02796-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Overexpression of either SH3PXD2A or CCR7 partially rescues the invasion and migration abilities inhibited by overexpression SH3PXD2A-AS1 in HTR8/SVneo cells. Left: cells stained with crystal violet after Transwell assays; right: quantification of cells with invasion or migration for Transwell assays. b Knockout of SH3PXD2A-AS1 partially rescues the invasion and migration abilities inhibited by either knockdown of SH3PXD2A or CCR7 in HTR8/SVneo cells. Left: cells stained with crystal violet after Transwell Assays; Right: quantification of cells with invasion or migration for Transwell assays. c SH3PXD2A-AS1 overexpression inhibited the expression of marker genes of invasion and migration in HTR8/SVneo cells. Left: results of qPCR; right: results of western blot. d SH3PXD2A-AS1 knockout promoted the expression of marker genes of invasion and migration in HTR8/SVneo cells. Left: results of qPCR; right: results of western blot. e The upregulation of marker genes of invasion and migration in cells with SH3PXD2A overexpression. Left: results of qPCR; right: results of western blot. f The upregulation of marker genes of invasion and migration in cells with CCR7 overexpression. Left: results of qPCR; right: results of western blot. g The downregulation of marker genes of invasion and migration in cells with SH3PXD2A knockdown. Left: results of qPCR; right: results of western blot. h The downregulation of marker genes of invasion and migration in cells with CCR7 knockdown. Left: results of qPCR; right: results of western blot. **p < 0.01, *p < 0.05.