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. Author manuscript; available in PMC: 2021 Apr 21.
Published in final edited form as: Anal Chem. 2020 Apr 7;92(8):5986–5993. doi: 10.1021/acs.analchem.0c00221

Figure 4.

Figure 4.

(a) HCD (NCE 25) and (b) UVPD (10 pulses, 2.5 mJ) spectra of m/z 728.52 identified as PE 17:1(c9Δ)_18:1(11Δ) from the chromatographic separation of an E. coli lipid extract. (c) Combined HCD and UVPD fragment ion map for m/z 728.52. (d) MS3 CID/UVPD spectrum of the m/z 464.27 fragment ion acquired from direct infusion of an E. coli lipid extract. (e) Fragment ion map for CID/UVPD of m/z 464.27. Diagnostic UVPD double bond and cyclopropane ions are labeled with red and green arrows in spectra, respectively, and red and green markers in the fragment ion maps, respectively. MS3 analysis confirms a cyclopropane in 17:1 and therefore a double bond in 18:1.