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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Cancer Res. 2020 Apr 20;80(13):2833–2847. doi: 10.1158/0008-5472.CAN-19-2733

Figure 3.

Figure 3.

ΔNp63 re-expression is required for lung extravasation and colonization of breast cancer MCF10DCIS cells. A, Experimental design to investigate roles of ΔNp63 in extravasation (48 hours) and colonization (8 weeks) to the lungs of mice that have been treated with MCF10DCIS cells expressing an inducible shRNA against ΔNp63. B-C, Flow cytometry assay (B) and quantification (C) of the percentage of RFP-positive MCF10DCIS cells in the lungs. Data are mean ± SD. n = 6. Asterisk indicates p < 0.05. D-E, Representative hematoxylin and eosin (H&E) cross-sections of lungs from the indicated groups (D) and quantification of metastatic colonies per lung section per mouse (E). Data are mean ± SD. n = 4 (group 1 and group 2) or n = 5 (group 3). Asterisk indicates p < 0.0005.