Table 1.
Relative Ratios of Metabolite Concentrations Under Normoxia and Hypoxia (1% O2 and 5% CO2).
| Metabolite | Normoxia | Hypoxia | Metabolite | Normoxia | Hypoxia | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Average | SD | CV | Average | SD | CV | Average | SD | CV | Average | SD | CV | ||
| Glycolic acid | 1.00 | 0.09 | 0.09 | 1.74 | 0.55 | 0.32 | Gly | 1.00 | 0.02 | 0.02 | 1.01 | 0.03 | 0.03 |
| Pyruvic acid | 1.00 | 0.10 | 0.10 | 1.89 | 0.15 | 0.08 | Putrescine | 1.00 | 0.16 | 0.16 | 0.33 | 0.03 | 0.09 |
| Lactic acid | 1.00 | 0.12 | 0.12 | 3.02 | 0.26 | 0.09 | β-Ala | 1.00 | 0.05 | 0.05 | 0.68 | 0.03 | 0.05 |
| Fumaric acid | 1.00 | 0.07 | 0.07 | 0.75 | 0.02 | 0.02 | Ala | 1.00 | 0.04 | 0.04 | 0.89 | 0.03 | 0.03 |
| 2-Oxoisovaleric acid | 1.00 | 0.13 | 0.13 | 4.39 | 0.36 | 0.08 | γ-Aminobutyric acid | 1.00 | 0.05 | 0.05 | 0.33 | 0.01 | 0.03 |
| Succinic acid | 1.00 | 0.16 | 0.16 | 1.54 | 0.12 | 0.08 | Ser | 1.00 | 0.06 | 0.06 | 2.58 | 0.05 | 0.02 |
| Malic acid | 1.00 | 0.07 | 0.07 | 1.03 | 0.05 | 0.04 | Cytosine | 1.00 | 0.12 | 0.12 | 0.75 | 0.03 | 0.03 |
| 2-Oxoglutaric acid | 1.00 | 0.11 | 0.11 | 1.36 | 0.15 | 0.11 | Uracil | 1.00 | 0.16 | 0.16 | 0.68 | 0.02 | 0.04 |
| Phosphoenolpyruvic acid | 1.00 | 1.23 | 1.23 | 0.61 | 0.15 | 0.24 | Creatinine | 1.00 | 0.00 | 0.00 | 0.88 | 0.02 | 0.02 |
| Dihydroxyacetone phosphate | 1.00 | 0.05 | 0.05 | 0.05 | 0.01 | 0.21 | Pro | 1.00 | 0.03 | 0.03 | 0.80 | 0.01 | 0.02 |
| Glycerol-3-phosphate | 1.00 | 0.07 | 0.07 | 2.04 | 0.28 | 0.14 | Val | 1.00 | 0.06 | 0.06 | 1.86 | 0.07 | 0.04 |
| cis-Aconitic acid | 1.00 | 0.05 | 0.05 | 0.31 | 0.02 | 0.06 | Homoserine | 1.00 | 0.18 | 0.18 | 1.20 | 0.20 | 0.17 |
| 3-Phosphoglyceric acid | 1.00 | 0.09 | 0.09 | 1.21 | 0.08 | 0.06 | Thr | 1.00 | 0.03 | 0.03 | 1.34 | 0.07 | 0.05 |
| Citric acid | 1.00 | 0.09 | 0.09 | 0.33 | 0.07 | 0.22 | Cys | 1.00 | 0.15 | 0.15 | 1.54 | 0.07 | 0.05 |
| Isocitric acid | 1.00 | 0.03 | 0.03 | 0.24 | 0.03 | 0.12 | Hydroxyproline | 1.00 | 0.02 | 0.02 | 0.79 | 0.03 | 0.04 |
| Gluconic acid | 1.00 | 0.07 | 0.07 | 0.81 | 0.07 | 0.09 | Creatine | 1.00 | 0.09 | 0.09 | 0.70 | 0.01 | 0.02 |
| Erythrose 4-phosphate | 1.00 | 0.19 | 0.19 | 0.29 | 0.02 | 0.07 | Ile | 1.00 | 0.04 | 0.04 | 1.21 | 0.02 | 0.02 |
| Ribose 5-phosphate | 1.00 | 0.03 | 0.03 | 0.75 | 0.07 | 0.09 | Leu | 1.00 | 0.02 | 0.02 | 1.33 | 0.07 | 0.05 |
| Ribulose 5-phosphate | 1.00 | 0.11 | 0.11 | 0.11 | 0.04 | 0.35 | Asn | 1.00 | 0.03 | 0.03 | 0.82 | 0.03 | 0.03 |
| Fructose 6-phosphate | 1.00 | 0.07 | 0.07 | 0.35 | 0.04 | 0.11 | Ornithine | 1.00 | 0.05 | 0.05 | 2.99 | 0.33 | 0.11 |
| Glucose 6-phosphate | 1.00 | 0.26 | 0.26 | 0.95 | 0.06 | 0.06 | Asp | 1.00 | 0.12 | 0.12 | 0.74 | 0.05 | 0.07 |
| Glucose 1-phosphate | 1.00 | 0.06 | 0.06 | 0.27 | 0.03 | 0.12 | Adenine | 1.00 | 0.16 | 0.16 | 1.44 | 0.25 | 0.18 |
| 6-Phosphogluconic acid | 1.00 | 0.12 | 0.12 | 1.02 | 0.10 | 0.10 | Anthranilic acid | 1.00 | 0.08 | 0.08 | 0.34 | 0.17 | 0.51 |
| Sedoheptulose 7-phosphate | 1.00 | 0.10 | 0.10 | 0.77 | 0.02 | 0.02 | Tyramine | 1.00 | 0.08 | 0.08 | 1.12 | 0.06 | 0.05 |
| dTMP | 1.00 | 0.26 | 0.26 | 1.15 | 0.34 | 0.30 | Spermidine | 1.00 | 0.23 | 0.23 | 0.81 | 0.40 | 0.49 |
| CMP | 1.00 | 0.16 | 0.16 | 0.29 | 0.02 | 0.08 | Gln | 1.00 | 0.01 | 0.01 | 4.64 | 0.08 | 0.02 |
| Fructose 1,6-diphosphate | 1.00 | 0.04 | 0.04 | 0.08 | 0.01 | 0.15 | Lys | 1.00 | 0.03 | 0.03 | 2.10 | 0.12 | 0.06 |
| AMP | 1.00 | 0.35 | 0.35 | 0.15 | 0.02 | 0.11 | Glu | 1.00 | 0.04 | 0.04 | 1.02 | 0.00 | 0.00 |
| IMP | 1.00 | 0.41 | 0.41 | 0.06 | 0.00 | 0.06 | Met | 1.00 | 0.04 | 0.04 | 1.50 | 0.09 | 0.06 |
| GMP | 1.00 | 0.17 | 0.17 | 0.18 | 0.02 | 0.11 | Guanine | 1.00 | 0.43 | 0.43 | 0.34 | 0.09 | 0.28 |
| NADPH | 1.00 | 0.09 | 0.09 | 0.43 | 0.05 | 0.12 | His | 1.00 | 0.02 | 0.02 | 1.39 | 0.08 | 0.05 |
| PRPP | 1.00 | 0.21 | 0.21 | 4.66 | 1.09 | 0.23 | Phe | 1.00 | 0.05 | 0.05 | 1.87 | 0.09 | 0.05 |
| FAD | 1.00 | 0.06 | 0.06 | 1.03 | 0.17 | 0.16 | Arg | 1.00 | 0.04 | 0.04 | 1.28 | 0.09 | 0.07 |
| dTDP | 1.00 | 0.12 | 0.12 | 0.25 | 0.17 | 0.66 | Citrulline | 1.00 | 0.05 | 0.05 | 1.25 | 0.08 | 0.06 |
| CDP | 1.00 | 0.16 | 0.16 | 0.44 | 0.01 | 0.03 | Tyr | 1.00 | 0.03 | 0.03 | 1.53 | 0.04 | 0.03 |
| Acetyl-CoA | 1.00 | 0.10 | 0.10 | 6.36 | 0.27 | 0.04 | DOPA | 1.00 | 0.11 | 0.11 | 1.45 | 0.22 | 0.15 |
| Malonyl-CoA | 1.00 | 0.22 | 0.22 | 0.22 | 0.04 | 0.19 | Trp | 1.00 | 0.03 | 0.03 | 1.87 | 0.04 | 0.02 |
| ADP | 1.00 | 0.13 | 0.13 | 0.40 | 0.05 | 0.14 | Carnosine | 1.00 | 0.05 | 0.05 | 0.80 | 0.03 | 0.04 |
| Succinyl-CoA | 1.00 | 0.26 | 0.26 | 0.77 | 0.02 | 0.03 | Cytidine | 1.00 | 0.10 | 0.10 | 0.87 | 0.11 | 0.13 |
| GDP | 1.00 | 0.23 | 0.23 | 0.46 | 0.06 | 0.12 | Adenosine | 1.00 | 0.31 | 0.31 | 0.62 | 0.21 | 0.34 |
| dCTP | 1.00 | 0.07 | 0.07 | 0.88 | 0.11 | 0.12 | Guanosine | 1.00 | 0.09 | 0.09 | 0.03 | 0.01 | 0.39 |
| dTTP | 1.00 | 0.16 | 0.16 | 1.21 | 0.29 | 0.24 | Glutathione (GSSG) divalent | 1.00 | 0.33 | 0.33 | 0.83 | 0.23 | 0.28 |
| CTP | 1.00 | 0.07 | 0.07 | 0.87 | 0.05 | 0.05 | Glutathione (GSH) | 1.00 | 0.22 | 0.22 | 0.43 | 0.17 | 0.39 |
| UTP | 1.00 | 0.09 | 0.09 | 0.54 | 0.04 | 0.08 | S-Adenosylmethionine | 1.00 | 0.02 | 0.02 | 1.74 | 0.18 | 0.10 |
| dATP | 1.00 | 0.13 | 0.13 | 1.38 | 0.16 | 0.11 | |||||||
| ATP | 1.00 | 0.11 | 0.11 | 1.09 | 0.10 | 0.09 | |||||||
| GTP | 1.00 | 0.08 | 0.08 | 1.32 | 0.07 | 0.05 | |||||||
| NAD+ | 1.00 | 0.03 | 0.03 | 1.16 | 0.24 | 0.21 | |||||||
| NADH | 1.00 | 0.03 | 0.03 | 0.33 | 0.04 | 0.11 | |||||||
| NADP+ | 1.00 | 0.05 | 0.05 | 0.74 | 0.04 | 0.06 | |||||||
Abbreviation: CV, coefficient of variation.