Fig. 1. NgBR expression in NSCLC tissues and cell lines.
A, expression of NgBR protein was assessed in normal lung cells and NSCLC cell lines using Western blot. Level of β-actin was used as a loading control (left panel). The intensity of protein levels was quantified using the Image Lab 5.2.1 software and normalized to β-actin (right panel). Error bar, SD of three independent experiments. ***p < .001 and ****p < .0001 vs. normal cells. B, representative immunostained tissue microarray shows high NgBR expression in NSCLC tissues and the corresponding tumor cell-positive lymph nodes vs. the adjacent lung tissues. Scale bar, 20 or 200 μm. C and D, Immunostaining score of NgBR expression in 52 cases of NSCLC tissues, their corresponding adjacent lung tissues, and tumor cell-positive lymph nodes. ****p < .0001 vs. normal tissues.