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. Author manuscript; available in PMC: 2020 Jul 28.
Published in final edited form as: Cancer Lett. 2018 Jan 11;418:135–146. doi: 10.1016/j.canlet.2018.01.030

Fig. 2. NgBR regulates EMT in NSCLC cell lines.

Fig. 2.

A, expression of NgBR, E-cadherin, and Vimentin in NSCLC cells using Western blot analysis. β-actin was used as a loading control. B, A549 cells were transfected with plRES-NgBR (NgBR) or plRES-NC (NC) for 72 h and subjected to Western blot analysis of different proteins. C, H1299 cells were transfected with NgBR siRNAs (SI, S2, and S3) or All-Star non-silencing siRNA (NS) for 72 h and then subjected to whole-cell lysate extraction and Western blot analysis. D and E, A549 cells were stably transfected with plRES-NC (NC) or plRES-NgBR (NgBR), while HI299 cells were stably transfected with NgBR shRNA (shNgBR) or nonspecific control (NC) and then subjected to Western blot analysis.