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. Author manuscript; available in PMC: 2020 Jul 28.
Published in final edited form as: Cancer Lett. 2018 Jan 11;418:135–146. doi: 10.1016/j.canlet.2018.01.030

Fig. 6. NgBR promotes Ras activation and plasma membrane localization in NSCLC cells.

Fig. 6.

A and B, cell membrane protein was extracted from stable A549 and H1299 cells to evaluate the localization of E-cadherin, K-Ras and H-Ras. Na, K ATPase and β-actin were used as markers for the membrane and cytoplasm, respectively. C, the complex of activated Ras was pulled down by GST-Raf1-RBD beads, and then assessed using Western blotting in A549 cells. Total protein levels were used as the negative control. D, the schematic illustration of NgBR regulation of NSCLC cell EMT.