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Follicles cultured in 3D medium: (A) day 1, (B) day 6, (C) day 12; Follicles cultured in 2D medium: (D) day 1, (E) day 6, (F) day 12. The 2D culture system is unable to conserve the spherical structure of follicles and the follicles adhere to the plate bottom. Granulosa cells break their basement membrane and distance from the oocyte (F), but the follicles encapsulated with sodium alginate can conserve their 3D follicular structure (C). Arrow mark antrum. Bar = 100 μm.
