Table 1.

Primers used in real-time PCR

Gene (Mus musculus)	Primer (5'-3')	Annealing temperature (°C)	Product size (bp)
	Forward: GTCCTACATCTGGCTGAAGTG	61	150
AMH	Reverse: GTCCAGGGTATAGCACTAACAG
		Forward: ATTGCGGAGCTGGAGATG	56	103
CYP11a1	Reverse: CTGGAAGTTGAAGAGGATGGG
		Forward: GAAGGGCAAGGACTTCTCTG	60	153
CYP17a1	Reverse: TGAACAGGGCAAAGGTGG
		Forward: AGCTGAGAAACTGGAAGACTG	59	108
CYP19a1	Reverse: GAAGTACAGAGTGACCGACATG
		Forward: GGGAAAGAGAAACACTGAACAC	60	169
GNRH	Reverse: TCTGCCATTTGATCCACCTC
		Forward: GATTACTGCTCTGGCTCCTAG	61	151
ACTB	Reverse: GACTCATCGTACTCCTGCTTG
	AMH: Anti-Müllerian hormone, CYP11a1: Cytochrome P450 Family 11 Subfamily A Member 1, CYP17a1; Cytochrome P450 Family 17 Subfamily A Member 1, CYP19a1; Cytochrome P450 Family 19 Subfamily A Member 1, GNRH: Gonadotropin-releasing hormone