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. Author manuscript; available in PMC: 2020 Jul 28.
Published in final edited form as: Nat Med. 2019 Oct 7;25(10):1607–1614. doi: 10.1038/s41591-019-0584-2

Extended Data Fig. 2 |. Preservation of rectal cancer histopathology in tumoroids.

Extended Data Fig. 2 |

a, Gross resected rectal specimen from which the first RC tumoroid line (RC-MSK-001) was derived and representative brightfield microscopy of the tumoroid in 3D culture two months after processing. Lower panels show hematoxylin and eosin (H&E) staining of the patient tumor (bottom left panel) and the derived RC-MSK-001 tumoroid (bottom right panel) in 3D culture. Scale bars, 50 μm. b, Hoechst and MitoTracker stains of a representative section of the RC-MSK-001 tumoroid demonstrate the luminal and glandular structure. Scale bars, 20 μm. c, Perineal recurrence of the original RC-MSK-001 tumor and the derived tumoroid (RC-MSK-001PR) are shown with H&E staining. Scale bar, 50 μm. d, H&E comparison of 32 additional tumoroid cell lines as noted with the corresponding primary tumor from which they were derived. Scale bars, 50 μm. All representative images are from one patient-specific tumor-to-tumoroid derivation.