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Genomic DNA isolated from fingernails show wild-type sequence for the JAK2 V617 locus. DNA was isolated and subjected to polymerase chain reaction amplification and Sanger sequencing. Shown are results from capillary electrophoretic sequencing in (A) forward and (B) reverse directions. The V617 corresponding DNA locus is highlighted in blue with only wild-type bases present in the forward and reverse direction.
