Fig. 4.

HSPA5 secondary structure did not change after insertion into liposomes. Circular dichroism performed for HSPA5-FL (5–10 μM) before and after incorporation in POPS or CL liposomes (3 mM) in 50 mM Tris-HCl buffer pH 7.5. A J-815 spectropolarimeter coupled to the Peltier system, at 25 °C, was used in these experiments. The secondary structures of HSPA5-FL APO and after incorporated in POPS or CL liposomes presented no differences, suggesting that incorporation of protein into liposomes had no effect in the secondary structure.