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CL liposomes aggregated in the presence of Hsp70s. The aggregation of POPS or CL liposomes with or without incorporation of HSPA5-FL (a, b) or HSPA1-FL (c, d) in 50 mM Tris-HCl 50 buffer (pH 7.5) was monitored by the changes in absorbance at 340 nm every 10 s for 1 h at 25 °C. a and c aggregation pattern. b and d visualization of aggregates in 96-well plates.
