Table 1.

UPLC conditions for melittin analysis

Item	Condition
Column	Halo ES-C18(2.1 × 100 mm, 2.7 μm, Advanced materials technology, USA)
Flow rate	0.8 mL/min
Column temperature	50 °C
Injection volume	2 μl
Wavelength	220 nm
Mobile phase	Time (min)	20 mM TFAa/MeCN(%)	20 mM TFA/H2O(%)
	0	40	60
	6	45	55

^aTFA trifluoroacetic acid