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HIV-specific cell-mediated immunity induced by a single vaccination. All studies were conducted in eight mice per group without (left panel) or with (right panel) pre-existing anti-Ad5 neutralizing antibodies (induced by vaccination 8 weeks earlier with 5 × 108 pfu of Ad-Luc). HIV-specific cell-mediated immunity after a single vaccination was measured (A) using a tetramer binding assay and (B) using an ICCS assay. (C) HIV-specific IgG serum antibody levels were measured by ELISA
