Extended Data Fig. 6 |. FACS analysis of human LAD2 mast cell loading of ^SiahIgE and ^AshIgE, phenotypic staining of PBMC-derived mast cells, and activation in primary basophils.

a, MFI (left) and representative histogram (right) of surface-bound hIgE on LAD2 mast cells following sensitization with PBS, OVA-specific ^SiahIgE or ^AshIgE (n = 3 technical replicates per group). Data are mean ± s.e.m. and are representative of three independent experiments. One-way ANOVA with Tukey’s multiple comparison test. b, Representative phenotypic staining by FACS of primary human mast cells from peripheral blood-derived CD34⁺ pluripotent hematopoietic cells (n = 2 technical replicates per group). c, Gating strategy for basophil activation assay. Representative FACS plots used to determine basophil activation from PBMC.