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. 2020 Jul 21;8:577. doi: 10.3389/fcell.2020.00577

FIGURE 3.

FIGURE 3

BBB score, NeuN immunofluorescent staining, and TUNEL assay of the cerebral cortex motor area in Lentivirus-treated rats. (A) The expression of PDXK following PDXK ORF and PDXK shRNA transfection in the cortex of SCT rats by Western blot. (B) The locomotor function was assessed by BBB score. (C) Pictures of neurons collected by double immunofluorescent staining of NeuN and PDXK in the cerebral cortex motor area. (D) Quantitative column diagram of number of NeuN and PDXK double-positive cells was obtained by Leica AF6000 system. Scale bar = 50 μm. *P < 0.05; **P < 0.01. (E) Pictures of apoptotic cells measured by NeuN immunofluorescent staining and TUNEL staining, presented by NeuN + Tunel + /NeuN + (%) in the cerebral cortex motor by Leica AF6000 system. (F) Quantitative histogram of neuronal apoptosis. Scale bar = 50 μm. *P < 0.05; **P < 0.01. Data were exhibited as mean ± SD. BBB, Basso, Beattie, and Bresnehan; TUNEL, terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick-end labeling; PDXK, pyridoxal kinase; SCT, spinal cord transection; DAPI, 4, 6-diamidino-2-phenylindole.