Figure 3.

AMMO1 Cross-reacts with rhLCV gH/gL

(A and B) AMMO1 conjugated to Dylite-650 was used to stain (A) untransfected 293T cells or (B) 293T cells transfected with plasmids expressing rhLCV gH and rhLCV gL. The scale bar represents 400 μm. The fluorescence channel for Dylite-650 (shown in blue) is overlaid on the transmitted light channel (gray). Images are representative of two independent experiments.

(C–F) Rhesus PBMC (C) or rhesus PBMC challenged with RhLCV alone (D) or rhLCV pre-incubated with AMMO1 (E) or AMMO2 (F) were cultured in medium. 3 days later, B cells (live, CD19⁺, CD20⁺) were monitored for EBNA2 expression by flow cytometry. Values indicate the percentage of EBNA2⁺ B cells.

(G) CHO-K1 cells were transfected with expression plasmids encoding rhLCV gH, rhLCV gL, rhLCV gB, and luciferase under control of a T7 promoter. 24 h later, the cells were trypsinized and then overlaid on HEK293 cells stably expressing T7 polymerase in wells containing AMMO1, CL59, CL40, E1D1, or no mAb, as indicated. 24 h later, the cells were lysed and assayed for luciferase activity. As a control for non-specific fusion, CHOK1 cells were transfected as above, except the plasmid rhLCV gB was replaced with an empty plasmid (delta gB).

Significant differences (∗p ≤ 0.05 and ∗∗p ≤ 0.01) were determined using an unpaired 2-tailed t test.