Skip to main content
. Author manuscript; available in PMC: 2021 Jun 8.
Published in final edited form as: Dev Cell. 2020 Jun 8;53(5):561–576.e9. doi: 10.1016/j.devcel.2020.05.007

Figure 3. Temporospatial Chromatin Looping of the Chr27 β-Keratin Gene Cluster during Skin Development.

Figure 3.

(A) Schematic of NG Capture-C experiment and its data presentation.

(B) Schematic of selected H3K27ac regions as candidate looping anchors at the Chr27 β-krt cluster. Red lines, selected H3K27ac regions; pink bars and blue triangles, bait sites (viewpoints) used in NG Capture-C experiments.

(C) Schematic of experimental design for comparison of skin “macro” and “micro” differences.

(D) NG Capture-C interactions of different skin regions (feather filaments and scale epidermis) from the same-aged chicks (E14).

(E) NG Capture-C interactions of different feather types (dorsal contour feather barbs and flight feather barbs) from the same-aged adult chickens.

(F) NG Capture-C interactions of different aged skins (E7, E14, and adult) from the same dorsal back region. Green triangles, feather-specific chromatin interactions; red triangles, adult contour feather-specific chromatin interactions.

(F′)Schematic of dynamic intra-cluster chromatin looping of Chr27 β-krt cluster during feather-skin development.

(G) NG Capture-C interactions of FK-TE at the Chr25 β-krt cluster.

(G′) Schematic of dynamic and inter-subcluster chromatin looping at the Chr25 β-krt cluster.