Skip to main content
. Author manuscript; available in PMC: 2021 Jun 8.
Published in final edited form as: Dev Cell. 2020 Jun 8;53(5):561–576.e9. doi: 10.1016/j.devcel.2020.05.007

Figure 4. β-Keratin Expression Patterns Are the Same for Those within Feather Subclusters on Chr25 or Co-expressed for Those within the Same Chromatin Loops on Chr27.

Figure 4.

(A) Expression of Chr25 β-krt genes in embryonic scale (left column), embryonic feather (middle column), and adult feather (right column). First row, H&E staining. Second row, common β-krt. Third to fifth rows, Scale Krt 18, FK3, and FK12 from Chr25, respectively. Note FK3 and FK12 show the same expression pattern. (A′) Schematic drawing of single enhancer-driven co-expression of subclustered genes on the Chr25 β-krt cluster.

(B) Expression of Chr27 FK genes in embryonic (left column) and adult feathers (right column). Note FK1 and FK12 show the same expression pattern in both embryonic and adult feathers, whereas FK39 and FK45 show the same expression pattern in embryonic feathers and different patterns in adult feathers.

(B′) Schematic drawing of intra-cluster chromatin looping of Chr27 β-krt cluster.

(C and D) Schematic of the two-mode epigenetic regulation – enhancer-driven co-expression of the whole subclustered FK genes on the Chr25 β-krt cluster (C) or CTCF/KLF4-mediated temporospatial chromatin looping of the Chr27 β-krt cluster (D).