REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E. coli BL21(DE3)	NEB	Cat.# C2527I
E. coli BL21-CodonPlus	Agilent	Cat.# 230280
E. coli Rosetta (DE3)	Novagen	Cat.# 70954-3
SARS-CoV-2 infected Vero E6 cells	Blanco-Melo et al., 2020	N/A
Chemicals, Peptides, and Recombinant Proteins
3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate	Anatrace	Cat.# C317
Adenosine 5′-diphosphate sodium salt	Sigma-Aldrich	Cat.# A2754-1G
Aluminum fluoride	Sigma-Aldrich	Cat.# 449628-10G
LDH/PK	Sigma-Aldrich	Cat.# P0294-5ML
NADH	Sigma-Aldrich	Cat.# N7410-15VL
PEP	Sigma-Aldrich	Cat.# P7127-250MG
SARS-CoV-2 nsp12 (biochemistry and cryo-EM)	This paper	N/A
SARS-CoV-2 nsp13 (biochemistry and cryo-EM)	This paper	N/A
SARS-CoV-2 nsp7/8 (biochemistry and cryo-EM)	This paper	N/A
SuperScript III Reverse Transcriptase	Invitrogen	Cat.# 18080051
Critical Commercial Assays
Kinase-Glo Plus Assay Kit	Promega	Cat.# V3771
Deposited Data
Coordinates of SARS-CoV-2 nsp132-RTC	This paper	PDB: 6XEZ
Cryo-EM map of SARS-CoV-2 (nsp132-RTC)2	This paper	EMD-22271
Cryo-EM map of SARS-CoV-2 nsp131-RTC	This paper	EMD-22270
Cryo-EM map of SARS-CoV-2 nsp132-RTC	This paper	EMD-22160
Repository of raw data	This paper	https://data.mendeley.com/datasets/njf7w7m5js/draft?a=8a8a4e75-3e95-41f5-9d90-6956c34b5aa3
Oligonucleotides
The oligonucleotides used in this study are listed in Table S2.	This paper	N/A
Recombinant DNA
pCDFDuet-1	Novagen	Cat.# 71340-3
pCDFDuet-1-His6-SARS-CoV-2-nsp7_nsp8	This paper	N/A
pET28-His6-SARS-CoV-2-nsp13	GenScript	N/A
pRSFDuet-1	Novagen	Cat.# 71341-3
pRSFDuet-1-His6-SUMO-SARS-CoV-2-nsp12	This paper	N/A
Software and Algorithms
blocfilt	Cardone et al., 2013	https://lsbr.niams.nih.gov/bsoft/programs/blocfilt.html
blocres	Cardone et al., 2013	https://lsbr.niams.nih.gov/bsoft/programs/blocres.html
Coot	Emsley and Cowtan, 2004	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot
cryoSPARC	Punjani et al., 2017	https://cryosparc.com/
Dali server	Holm and Laakso, 2016	http://ekhidna2.biocenter.helsinki.fi/dali/
EMBL-EBI Clustal Omega	Madeira et. Al., 2019	https://www.ebi.ac.uk/Tools/msa/clustalo/
GraphPad Prism	GraphPad	http://www.graphpad.com/scientific-software/prism/
Leginon	Suloway et al., 2005	https://emg.nysbc.org/redmine/projects/leginon/wiki/Leginon_Homepage
Molprobity	Chen et al., 2010	http://molprobity.biochem.duke.edu
MotionCor2	Zheng et al., 2017	https://emcore.ucsf.edu/ucsf-software
mtriage	Afonine et al., 2018	https://www.phenix-online.org/documentation/reference/mtriage.html
PHENIX	Adams et al., 2010	https://www.phenix-online.org/documentation/index.html
RELION	Scheres, 2012	https://www2.mrc-lmb.cam.ac.uk/relion
RELION Bayesian Polishing	Zivanov et al., 2018	https://www2.mrc-lmb.cam.ac.uk/relion
SBGrid	Morin et al., 2013	https://sbgrid.org/
The PyMOL Molecular Graphics System	Schrödinger, LLC	https://pymol.org/2/
UCSF Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera
UniDec	Marty et. al., 2015	https://github.com/michaelmarty/UniDec/releases
WebLogo	Schneider and Stephens, 1990	https://weblogo.berkeley.edu/
Other
C-flat CF-1.2/1.3 400 mesh gold grids	Protochips, Inc.	Cat.# CF413-100-Au
cOmplete Protease Inhibitor Cocktail	Roche	Cat.# 11697498001
Gibson Assembly Master Mix	NEB	Cat.# E2611S
HiLoad 16/600 Superdex 200 pg	GE Healthcare Life Sciences	Cat.# GE28-9893-3
HiLoad 16/600 Superdex 75 pg	GE Healthcare Life Sciences	Cat.# GE28-9893-33
HisTrap HP	GE Healthcare Life Sciences	Cat.# GE17-5248-02
HiTrap Heparin HP	GE Healthcare Life Sciences	Cat.# GE17-0407-03
Isotope [α-32P] ATP	Perkin Elmer	Cat.# BLU003H250UC
Superose 6 Increase 10/300 GL column	GE Healthcare Life Sciences	Cat.# GE29-0915-96
Zeba Spin Desalting Columns, 40K MWCO	Thermo Scientific	Cat.# 87765
Zeba Spin Desalting Columns, 7K MWCO	Thermo Scientific	Cat.# 89882