KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| SARS-CoV-2 S protein | This Study | N/A |
| Human ACE2 protein | This Study | N/A |
| 2x Laemmli sample buffer | Bio-Rad | Cat#161–0737 |
| Invitrogen NuPAGE 4 to 12%, Bis-Tris, Mini Protein Gel | Thermo Fisher Scientific | Cat#NP0321PK2 |
| Coomassie Brilliant Blue G-250 Dye | Thermo Fisher Scientific | Cat#20279 |
| Dithiothreitol | Sigma Aldrich | Cat#43815 |
| Iodoacetamide | Sigma Aldrich | Cat#I1149 |
| Trypsin | Promega | Cat#V5111 |
| Lys-C | Promega | Cat#V1671 |
| Arg-C | Promega | Cat#V1881 |
| Glu-C | Promega | Cat#V1651 |
| Asp-N | Promega | Cat#VA1160 |
| Endoglycosidase H | Promega | Cat#V4871 |
| PNGaseF | Promega | Cat#V4831 |
| Chymotrypsin | Athens Research and Technology | Cat#16-19-030820 |
| Alpha lytic protease | New England BioLabs | Cat#P8113 |
| 18O water | Cambridge Isotope Laboratories | OLM-782-10-1 |
| O-protease OpeRATOR | Genovis | Cat#G1-OP1–020 |
| Deposited Data | ||
| MS data for site-specific N-linked glycopeptides for SARS-Cov-2 S and human ACE2 | This Study | PXD019937 |
| MS data for site-specific O-linked glycopeptides for SARS-Cov-2 S and human ACE2 | This Study | PXD019940 |
| MS data for deglycosylated N-linked glycopeptides for SARS-Cov-2 S and human ACE2 | This Study | PXD019938 |
| MS data for disulfide bond analysis for SARS-Cov-2 S | This Study | PXD019939 |
| MS data for N-linked glycomics deposited at GlycoPost | This Study | GPST000120 |
| MS data for O-linked glycomics deposited at GlycoPost | This Study | GPST000121 |
| Experimental Models: Cell Lines | ||
| 293-F Cells | Gibco | Cat#R79007 |
| Experimental Models: Organisms/Strains | ||
| VSV(G)-Pseudoviruses | This Study | |
| Software and Algorithms | ||
| pGlyco | (43) | v2.2.2 |
| Proteome Discoverer | Thermo Fisher Scientific | v1.4 |
| Byonic | Protein Metrics Inc. (44) | v3.8.13 |
| ProteoIQ | Premier Biosoft | v2.7 |
| GRITS Toolbox | http://www.gnts-toolbox.org (40) | v1.1 |
| EMBOSS needle | (61) | v6.6.0 |
| Biopython | https://biopython.org/ | v1.76 |
| Rpdb | https://rdrr.io/cran/Rpdb/ | v2.3 |
| SignalP | http://www.cbs.dtu.dk/services/SignalP/ (34) | v5.0 |
| LibreOFFICE Writer | The Document Foundation | V6.4.4.2 |
| GlyGen | https://www.glygen.org | V1.5 |
| GNOme | https://github.com/glygen-glycan-data/GNOme/blob/master/README.md | V1.5.5 |
| GlyTouCan | https://glytoucan.org | V3.1.0 |
| Inkscape | Inkscape Developers | V1.0 |